排序方式: 共有44条查询结果,搜索用时 15 毫秒
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Alice Franchi Marco Pedrazzi Alessandro Alberto Casazza Enrico Millo Gianluca Damonte Annalisa Salis Nara Liessi Franco Onofri Antonella Marte Silvia Casagrande Roberta De Tullio Patrizia Perego Monica Averna 《Molecules (Basel, Switzerland)》2020,25(19)
We have recently demonstrated that bioactive molecules, extracted by high pressure and temperature from olive pomace, counteract calcium-induced cell damage to different cell lines. Here, our aim was to study the effect of the same extract on murine cortical neurons, since the preservation of the intracellular Ca2+-homeostasis is essential for neuronal function and survival. Accordingly, we treated neurons with different stimuli in order to evoke cytotoxic glutamatergic activation. In these conditions, the high-pressure and temperature extract from olive pomace (HPTOPE) only abolished the effects of N-methyl-d-aspartate (NMDA). Particularly, we observed that HPTOPE was able to promote the neuron rescue from NMDA-induced cell death. Moreover, we demonstrated that HPTOPE is endowed with the ability to maintain the intracellular Ca2+-homeostasis following NMDA receptor overactivation, protecting neurons from Ca2+-induced adverse effects, including aberrant calpain proteolytic activity. Moreover, we highlight the importance of the extraction conditions used that, without producing toxic molecules, allow us to obtain protecting molecules belonging to proanthocyanidin derivatives like procyanidin B2. In conclusion, we can hypothesize that HPTOPE, due to its functional and nontoxic properties on neuronal primary culture, can be utilized for future therapeutic interventions for neurodegeneration. 相似文献
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Katja S. Hheim Emil Lindbck Kah Ni Tan Marte Albrigtsen Ida T. Urdal Helgeland Clmence Lauga Thodora Matringe Emily K. Kennedy Jeanette H. Andersen Vicky M. Avery Magne O. Sydnes 《Molecules (Basel, Switzerland)》2021,26(11)
A series of novel quinoline-based tetracyclic ring-systems were synthesized and evaluated in vitro for their antiplasmodial, antiproliferative and antimicrobial activities. The novel hydroiodide salts 10 and 21 showed the most promising antiplasmodial inhibition, with compound 10 displaying higher selectivity than the employed standards. The antiproliferative assay revealed novel pyridophenanthridine 4b to be significantly more active against human prostate cancer (IC50 = 24 nM) than Puromycin (IC50 = 270 nM) and Doxorubicin (IC50 = 830 nM), which are used for clinical treatment. Pyridocarbazoles 9 was also moderately effective against all the employed cancer cell lines and moreover showed excellent biofilm inhibition (9a: MBIC = 100 µM; 9b: MBIC = 100 µM). 相似文献
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Dr. Marte S. M. Holmsen David Vesseur Dr. Yago García-Rodeja Dr. Karinne Miqueu Dr. Didier Bourissou 《Angewandte Chemie (International ed. in English)》2023,62(31):e202305280
The possibility for AuIII σ-cyclopropyl complexes to undergo ring-opening and give π-allyl complexes was interrogated. The transformation was first evidenced within (P,C)-cyclometalated complexes, it occurs within hours at −50 °C. It was then generalized to other ancillary ligands. With (N,C)-cyclometalated complexes, the rearrangement occurs at room temperature while it proceeds already at −80 °C with a dicationic (P,N)-chelated complex. Density Functional Theory (DFT) calculations shed light on the mechanism of the transformation, a disrotatory electrocyclic ring-opening. Intrinsic Bond Orbital (IBO) analysis along the reaction profile shows the cleavage of the distal σ(CC) bond to give a π-bonded allyl moiety. Careful inspection of the structure and bonding of cationic σ-cyclopropyl complexes support the possible existence of C−C agostic interactions at AuIII. 相似文献
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Marte Balchen Trine Grønhaug HalvorsenLéon Reubsaet Stig Pedersen-Bjergaard 《Journal of chromatography. A》2009,1216(41):6900-6905
The present study has for the first time demonstrated the isolation of peptides from human plasma by electromembrane extraction (EME). Angiotensin 1, angiotensin 2, and angiotensin 3 migrated from 500 μL of diluted plasma, through a thin layer of 1-octanol and 8% di-(2-ethylhexyl) phosphate immobilized as a supported liquid membrane (SLM) in the pores of a porous hollow fiber, and into a 25 μL aqueous acceptor solution present inside the lumen of the fiber. The driving force for the extraction was a 15 V potential difference applied across the SLM. After only 10 min of EME, the peptides were isolated from diluted plasma (pH 3) with extraction recoveries between 25 and 43%. After optimization, the extraction system was evaluated using spiked plasma samples of angiotensin 2. The evaluation was performed by liquid chromatography electrospray mass spectrometry, showing linearity of angiotensin 2 in the range 2.5–125.0 ng/mL (r2 = 0.989), and repeatability (RSD) between 5.6 and 11.6% (n = 6). The results demonstrate the possibility of isolating angiotensin peptides from plasma in only 10 min, using electromembrane extraction. The experimental findings are therefore promising with regard to future peptide extractions. 相似文献
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Background
Recently several studies have shown that people use contextual information to make predictions about the rest of the sentence or story as the text unfolds. Using event related potentials (ERPs) we tested whether these on-line predictions are based on a message-level representation of the discourse or on simple automatic activation by individual words. Subjects heard short stories that were highly constraining for one specific noun, or stories that were not specifically predictive but contained the same prime words as the predictive stories. To test whether listeners make specific predictions critical nouns were preceded by an adjective that was inflected according to, or in contrast with, the gender of the expected noun. 相似文献39.
Molecules are created from a Bose-Einstein condensate of atomic 87Rb using a Feshbach resonance. A Stern-Gerlach field is applied, in order to spatially separate the molecules from the remaining atoms. For detection, the molecules are converted back into atoms, again using the Feshbach resonance. The measured position of the molecules yields their magnetic moment. This quantity strongly depends on the magnetic field, thus revealing an avoided crossing of two bound states at a field value slightly below the Feshbach resonance. This avoided crossing is exploited to trap the molecules in one dimension. 相似文献
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Dr. Marte Sofie Martinsen Holmsen Dr. Ainara Nova Dr. Sigurd Øien-Ødegaard Dr. Richard H. Heyn Prof. Mats Tilset 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2020,132(4):1532-1536
A highly asymmetric AuIII η3-allyl complex has been generated by treating Au(η1-allyl)Br(tpy) (tpy=2-(p-tolyl)pyridine) with AgNTf2. The resulting η3-allyl complex has been characterized by NMR spectroscopy and X-ray crystallography. DFT calculations and variable temperature 1H NMR suggest that the allyl ligand is highly fluxional. 相似文献